Prevalence of Hepatitis C virus Genotype 3a in patients with Hodgkin and Non-Hodgkin Lymphoma
Radmehr، Hashem نويسنده Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Radmehr, Hashem , Makvandi، Manoochehr نويسنده , , Samarbafzadeh، Alireza نويسنده , , Teimoori، Ali نويسنده Department of Virology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Neisi، Niloofar نويسنده , , Rasti، Mojtaba نويسنده Department of Medical Microbiology , School of Medicine , Ahvaz Jundishapur University of Medical Science, Ahvaz, IR Iran Rasti, Mojtaba , Abasifar، Sara نويسنده Virology Department, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Abasifar, Sara , Soltani، Hasan Ali نويسنده , , Abbasi، Samaneh نويسنده , , Kiani، Hadis نويسنده Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Kiani, Hadis , Mehravaran، Hamide نويسنده Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Mehravaran, Hamide , Azaran، Azarakhsh نويسنده Virology Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Shahani، Toran نويسنده Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Shahani, Toran
Background and Objectives: Hepatitis C virus (HCV) is a major public health problem worldwide. Replication and persistence of HCV genome have been described in the liver tissue as well as B cells lymphocyte. Several investigations have reported that long-term persistence of HCV in B cells may result in Hodgkin and Non-Hodgkin lymphoma. This study was aimed to determine frequency of HCV RNA in histological tissues obtained from patients suffered from Hodgkin and Non-Hodgkin lymphoma.
Materials and Methods: 52 formalin-fixed paraffin-embedded tissue blocks including 23 (44.3%) Hodgkin and 29 (55.7%) Non-Hodgkin samples were collected and five micrometer sections were prepared. RNA was extracted and cDNA was synthesized. Two consecutive Nested RT-PCR assays were carried out for detection of HCV 5’ UTR and core gene. RT-PCR products were sequenced and aligned to construct HCV phylogenic tree to evaluate the homology of sequences in comparison to the reference sequences retrieved from Genbank.
Results: Overall, 6 Non-Hodgkin (20.6%) and 3 Hodgkin lymphoma (13.04%) samples showed positive PCR results for both 5’ UTR and HCV core RNA via nested PCR (P<0.469). Sequencing results revealed that all detected HCV RNA samples belonged to the genotype 3a.
Conclusion: Despite low prevalence of HCV infection in Iran, high frequency of HCV RNA genotypes 3a (17.3%) has been found in patients with Hodgkin and Non-Hodgkin lymphoma. To improve treatment regimens, screening of HCV RNA in patients suffered from Hodgkin or Non-Hodgkin lymphoma is recommended which can be done through highly sensitive molecular means before and after immunosuppression status.
Keywords: Nested RT-PCR, Hepatitis C virus, Hodgkin lymphoma, Non-Hodgkin lymphoma, Genotype