Title of article
COMMUNICATION: HEPATITIS B VIRUS PRECORE PROTEIN MODELING: AN EXPERIENCE FROM NEW DELHI, INDIA
Author/Authors
Sarma, Manash Pratim Department of Biotechnology - Assam down town University, Guwahati, Assam, India , Kalita, Partha Pratim Department of Biotechnology - Assam down town University, Guwahati, Assam, India
Issue Information
دوفصلنامه با شماره پیاپی سال 2015
Pages
6
From page
113
To page
118
Abstract
Hepatitis B virus (HBV) genome infecting hepatocellular carcinoma (HCC) cases with special emphasis on mutation has been widely studied across the globe. However there is a paucity of information in proteomic level. The current study was aimed to analyze the structure and chemical composition of a core protein of HBV found in a HCC patient from New Delhi using bioinformatics software’s. An HCC case was diagnosed as per EASL 2001, guidelines and viral DNA was isolated followed by amplification of the core gene. The amplicon was sequenced commercially from Macrogen Korea and the nucleotide sequence was converted to amino acids using DNA 2.0. The protein sequence was than analysed using RaptorX for its structure and chemical composition. A total of 90 hydrogen bonds, 8 helices and 4 turns were found in this current protein model. However there were no strands in the model. Stable Ramachandran map was viewed. Highest numbers of Leucine were observed in the proposed structure. A stable protein model is successfully proposed in this study.
Keywords
HBV , chronic liver disease , precore protein , modeling , Ramachandran plot
Journal title
Astroparticle Physics
Serial Year
2015
Record number
2406332
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