Title of article
Influence of the Compounds Used in Immunochromatographic Test-system on its Analytical Characteristics: Multi-factor Optimization of the System for Aflatoxin B1
Author/Authors
Urusov, A. E A.N. Bach Institute of Biochemistry of the Russian Academy of Sciences - Leninsky prospect 33 - 119071 Moscow, Russia , Zherdev, A. V A.N. Bach Institute of Biochemistry of the Russian Academy of Sciences - Leninsky prospect 33 - 119071 Moscow, Russia , Dzantiev, B. B A.N. Bach Institute of Biochemistry of the Russian Academy of Sciences - Leninsky prospect 33 - 119071 Moscow, Russia
Pages
9
From page
63
To page
71
Abstract
Analytical parameters of immunochromatographic test-systems, such as the detection limit and working range, depend on many factors. However, the process of choosing the concentration and composition of reagents applied to test strip membranes, and choosing the membranes themselves has not been systematically described for commercially produced tests or for scientific studies. Here, we present the results of a study to determine how the properties of the test system components, using mycotoxin aflatoxin B1 as an example, affect its analytical performance. A comparison of different membranes used for immunochromatography was performed as well as reagent concentrations and composition of intermolecular conjugates. Optimization of the immunochromatographic system indicated a limit of aflatoxin B1 detection equal to 0.2 ng/mL. The effectiveness of the system in testing plant extracts with minimal (twice) dilution prior to analysis was determined and the final content of methanol in the course of immunoassay was 35%.
Farsi abstract
پارامترهاي تحليلي سيستم تست ايمونوكروماتوگرافي مانند حد آشكار سازي و محدوده ي عملكرد به فاكتورهاي زيادي بستگي دارد. اگرچه، فرايند انتخاب غلظت و تركيب معرف ها روي نوارهاي تست غشايي به كار برده شد اما انتخاب غشاها براي تست هاي توليدي تجاري يا براي مطالعات علمي به طور سيستماتيك بيان نشده است. در اينجا، ما نتايج تحقيقي را كه چگونه مشخصات اجزاي سيستم تست، استفاده از سم قارچي آفلاتوكسين B1 به عنوان نمونه، بر عملكرد تحليلي آن تاثير ميگذارد را نشان مي دهيم. مقايسه غشاهاي مختلف استفاده شده براي ايمونوكروماتوگرافي همچنين غلظت هاي معرف و تركيب پيوندهاي بين مولكولي 0/25 مي باشد. كارايي سيستم در طرح مورد ng / mg انجام شد. بهينه سازي سيستم ايمونوكروماتوگرافي بيان مي كند كه حد آشكار سازي آفلاتوكسين B1 برابر آزمايش كه استخراج با حداقل رقيق سازي (2 مرتبه) پيش از آناليز انجام شد، تعيين شد و ميزان نهايي متانول در مرحله سنجش ايمني 35٪ بود.
Keywords
Aflatoxin B1 , Mycotoxin , Immunoassay , Immunodetection , Immunochromatography , Detection limit
Journal title
Astroparticle Physics
Serial Year
2015
Record number
2425899
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