Title of article
A SIMPLE and ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY
Author/Authors
IJour-Amir, M Dept - of Biochemistry - Bobo! Univ, of Med. Sciences , Rasaee, M.J Dept - of Biochemistty - Tarbiat-Modares Univ, Tehran , Qujeq, D Dept - of Biochemistry - Bobo! Univ, of Med. Sciences , Asadikaram, GIIR Dept - of Biochemistty - Rafsanjan Univ, of Med. Sciences , Frozandeh Moqhadam, M Dept - of Biochemistty - Tarbiat-Modares Univ, Tehran
Pages
5
From page
53
To page
57
Abstract
The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease of large scale production. In this study, proteins from egg yolk were extracted with acid and chloroform. The result of cellulose acetate electrophoresis at pH 9 showed some extra bands which were further purified with sodium sulfate precipitation (20%). The purified band was observed in the y globulin region. SDS-PAGE eletrophoresis of the product showed impurities which were removed by T-gel chromatography. Following that only H-chain-of immunoglobulin Y with a molecular weight of about 66 KD was observed. Using the above procedure, a yield of around 40.7 mg IgY / egg was obtained.
Keywords
IgY , T-Gel Chromatography , Egg Yolk
Journal title
Astroparticle Physics
Serial Year
2000
Record number
2434487
Link To Document