Isolation of Cryptosporidium parvum Oocyst From Infected Feces

Objective: Cryptosporidiosis is a parasitic disease caused by Cryptosporidium parvum, a widely distributed protozoan parasite, which infects both wild and domesticated animals, as well as humans, chiefly immunocompromised individuals. Since its diagnosis in the 1970s, Cryptosporidium has been attributed an increasingly important role in the neonatal diarrhea syndrome of humans and newborn ruminants. It is very important to diagnose C. parvum disease and isolate C. parvum oocysts for different scientific targets. Materials and Methods: In the present study different methods for isolation of C. parvum oocysts were examined and a suitable method for preparation and isolation of C. parvum oocysts from infected feces was defined. Based on this method C. parvum infected watery feces were gathered; same size of potassium dichromate added and stored at 4°C. Fecal specimens were washed and filtrated, respectively with 52, 100, 150 screens. Separated and filtered solution was centrifuged at 2500 rpm for 5 minutes. Results: In the present study different methods were examined and evaluated. The defined method of Lorenzo et al, was distinguished as the most suitable method for preparation and isolation of C. parvum oocysts from infected feces. Conclusion: This work describes the successful development of method for the recovery and isolation of Cryptosporidium oocysts in feces.