Title of article
A study on the assessment of DNA strand-breaking activity by silver and silica nanoparticles
Author/Authors
Vahdati, Ali R Islamic Azad University, Tonekabon , Sadeghi, Babak Department of Chemistry - Tonekabon Branch - Islamic Azad University Tonekabon, Tonekabon
Pages
3
From page
1
To page
3
Abstract
We measured the DNA strand-breaking activity of nanoparticle by agarose gel electrophoresis. Plasmid DNA was
mixed with nanosilver and nano-silica powders in an aqueous solution. Then the mixture was applied to 1% of
agarose gel electrophoresis at 75 V for 30 min. We used 300 nm of UV irradiation in order to visualize the DNA
bands. The DNA strand-breaking activity of nanomaterial was measured through measuring the reduction of
super-coiled DNA to circular form of DNA.
Keywords
Silver nanoparticles , DNA , Nanotechnology
Journal title
Astroparticle Physics
Serial Year
2013
Record number
2436416
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