Radical Scavenging Activities and TLC-Guided Bioautography of the SC-CO2 extract of Ganoderma applanatum

SC-CO2 extracts from the fruiting bodies of Ganoderma applanatum were characterized by reverse phase High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared Spectroscopy (FTIR); and its antioxidant compounds were determined through DPPH Radical Scavenging Assay and TLC-guided Bioautography. The extracts were found to be essential oils in 10 MPa and secondary metabolites in 20 & 30 MPa. DPPH Assay revealed that the SC-CO2 extracts have an antioxidant activity through the determination of its radical scavenging activity along with ascorbic acid which can be summarized in the order: 10 MPa < 30 MPa < 20 MPa < ascorbic acid. Results of TLCbioautography using 2.54 mM DPPH showed that the compounds have antioxidant property through the change in color of the extracts in the TLC plate, which confirms the DPPH Radical Scavenging Assay. These results suggest that the extracts serve as a source of compounds with radical scavenging activity that can be used as antioxidants aside from its traditional uses.