Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer

Objective: Detection of P53 gene mutation on its codon 248 between Iranian women with breast cancer by PCR-RFLP. Material and methods: DNA extraction of tumor tissues was performed in 53 patients with primary breast cancer. Designing primer for the sub-studying codon was performed with NTIVector software and detection of codon 248 mutation was done by Msp I PCR_RFLP. Results: The somatic mutations of P53 on codon 248 were observed in 11.3% (6 of 53) of our cases, in which 9.4% were heterozygote mutation and the rest were homozygote mutation. This ratio is the highest reported percentage in the world. Conclusion: This perusal indicates genotyping of P53 gene on codon 248 is important for Iranian women with breast cancer risk factors.