Title of article
Finding the existence of of Helicobacter pylori in springs of Chaharmahal and Bakhtiari Province, Iran by polymerase chain reaction
Author/Authors
Khoshdel, Abolfazl Shahrekord University of Medical Sciences , Kasiri, Karam Ali Shahrekord University of Medical Sciences , Vahedi Dehkordi, Sepideh Shahrekord University of Medical Sciences , Lotfizadeh, Masoud Social Health Determinants Research Center - Shahrekord University of Medical Sciences , Hosseinzadeh, Shahreyar Shahrekord University of Medical Sciences , Rabie, Ali Asghar Shahrekord University of Medical Sciences
Pages
6
From page
345
To page
350
Abstract
Background and aims: Helicobacter pylori is the primary cause of various diseases. This study was conducted to determine the existence of H. pylori in springs and surface water in Chaharmahal and Bakhtiari province.
Methods: In this study, 29 water samples were collected from three different locations of Zayanderud River, 24 springs, and two subterranean from June 2014 to August 2014 in Chaharmahal and Bakhtiari province. Samples were filtered and DNAs were extracted. Then, the existence of H. pylori DNA was tested by polymerase chain reaction (PCR) for 16S rRNA, glm and Cag genes.
Results: In the present study, the total infection rate of samples by H. pylori was 24.13%. H. pylori and DNA was detected in 100% of samples of Zayanderud River, 12.5% in springs of Dehcheshmeh in Farsan and Baram in Lordegan, and 50% in aqueducts.
Conclusion: These findings show the existence of H. pylori in springs and surface water in Chaharmahal and Bakhtiari province and additional evidence for waterborne transmission of H. pylori in some environments.
Keywords
Helicobacter pylori , Polymerase Chain Reaction , Water
Journal title
Astroparticle Physics
Serial Year
2016
Record number
2444526
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