Is sub-chronic exercise in Combination with medicinal nanoparticles a protective strategy against Doxorubicin-induced Hepatic oxidative stress and apoptosis in aging model rats?

Objective(s): Oxidative stress and apoptosis are the major side effects of doxorubicin (DOX) and the advantages accruing from exercise and some medicinal herbs in mitigation of these toxic side effects is well documented. But so far, the effects of exercise in combination with medicinal nanoparticles on oxidative stress and apoptosis signaling simultaneously, in liver tissue are unknown. Hence, we investigated whether Treadmill Running in combination with Nanocurcumin protects the liver tissue against these toxic side effects (oxidative stress and apoptosis) simultaneously of DOX treatment in aging rats induced by D-galactose. Materials and Methods: Fifty-six Wistar male rats received a daily injection of D-galactose (100 mg/kg/day, i.p.) then randomly assigned to 7 sub-groups. The training protocol included treadmill running progressively between 25 to 54 min/day and 15 to 20m/min, 5 days/week for six weeks. DOX (1 mgmL−1kg−1day−1) was administrated intraperitoneally for 15 days and Nanocurcumin was administrated orally for 2 weeks (100 mg/kg/day). Results: Nanocurcumin Consumption led to insignificant increase in SOD, MDA and insignificant decrease in AIF levels. Treadmill running led to insignificant increase in SOD and insignificant decrease in AIF and MDA levels. The combination of Treadmill running and Nanocurcumin led to significant decrease in MDA and insignificant increase in SOD and insignificant decrease in AIF levels. Conclusion: In conclusion, Treadmill running exercise and Nanocurcumin partly mitigates the toxic side effects of DOX treatment. But this amount of treatment does not play a required role against DOX-induced hepatic damage.