Antibacterial and Anti-biofilm Investigation of Electrospun PCL/gelatin/Lawsone Nano Fiber Scaffolds against Biofilm Producing Bacteria

The aim of this study was to evaluate the antibacterial and anti-biofilm activity of polycaprolactone-gelatin (PCL/GEL) nanofibers containing lawsone against four pathogenic bacteria. Different concentrations of lawsone (2-hydroxy-1,4-naphthoquinone) (1, 3, 5 and 10%) were incorporated into PCL/GEL nanofibers via electrospinning technique. Presence of lawsone in the scaffold was confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial and anti-biofilm activities of lawsone loaded scaffolds against clinically isolated Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Proteus mirabilis were investigated by disc diffusion method. All scaffolds were able to inhibit the growth of S. aureus and MRSA. Additionally, PCL/GEL/lawsone 10% had bactericidal efficacy against P. mirabilis. Obtained results of the biofilm formation assay revealed that PCL/GEL/lawsone 10% inhibited the biofilm formation of S. aureus, MRSA and P. mirabilis. Due to good antibacterial and anti-biofilm activities of lawsone containing scaffolds, PCL/GEL 10% can be a good candidate for application in wound dressing patches.