Hydrothermal Synthesis of Cobalt- Doped Hydroxyapatite Nanoparticles: Structure, Magnetic Behaviour, Bioactivity and Antibacterial Activity

This study focuses on the physical, magnetic, biological and antibacterial behavior of cobalt-doped hydroxyapatite (HAp) powder samples. Pure and cobalt- doped HAp nanoparticles were synthesized by hydrothermal method. Calcium nitrate, di- ammonium hydrogen phosphate and cobalt nitrate were used as precursor materials. The synthesized powders were characterized using x-ray diffraction pattern (XRD), fourier transform infrared spectroscopy (FTIR), 􀀫eld emission scanning electron microscopy (FESEM), vibrating sample magnetometer (VSM), raman spectroscopy as well as MTT assay and cell adhesion test. Disc diffusion method was used to investigate antibacterial activity of the samples. The results con􀀫rmed the substitution of Ca by Co ions in the HAp lattice. In addition, this substitution induced size reduction and morphology change in HAp particles. All cobalt substituted HAp powder samples displayed paramagnetic properties, as opposed to the diamagnetic behavior observed in the pure HAp samples. In addition, these nanoparticles exhibited cell adhesion, cell viability and antibacterial activity against S.aureus bacteria.