Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimized prefractionation method. Using reverse phase-high performance liquid chromatography eight fractions were separated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile of these fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically, two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expression of some proteins in A.Fumigatus. To accurately identify these proteins, further experiments are needed including Mass spectrometry analysis.