Title of article
Spermatogenesis after Transplantation of Adipose Tissue-Derived Stem Cells in Azoospermic Guinea Pigs: A Histological and Histomorphometric Study
Author/Authors
Hajihoseini, Mehrdokht Department of Biology - Fars Science and Research Branch - Islamic Azad University, Fars, Iran , Mehrabani, Davood Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Vahdati, Akbar Department of Biology - Shiraz Branch, Islamic Azad University, Shiraz, Iran , Hosseini, Ebrahim Department of Biology - Shiraz Branch, Islamic Azad University, Shiraz, Iran , Tamadon, Amin The Persian Gulf Marine Biotechnology Research Center - The Persian Gulf Biomedical Sciences Research Institute - Bushehr University of Medical Sciences, Bushehr, Iran , Dianatpour, Mehdi Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Rahmanifar, Farhad Department of Basic Sciences - School of Veterinary Medicine - Shiraz University, Shiraz, Iran
Pages
11
From page
1
To page
11
Abstract
Background: The purpose of this research was to determine histomorphometric changes in busulfan-induced azoospermia after transplantation of Adipose Tissue-Derived Stem Cells (AdSCs) in guinea pig. AdSCs were isolated from adipose tissue around the testes of guinea pigs and characterized for mesenchymal properties. Materials and Methods: Guinea pigs were allocated into three groups, including the control group without any intervention. To induce azoospermia, groups 2 and 3 received a dose of 40 mg/kg of busulfan with 21 days interval. Group 3 received 1×106 AdSCs in their seminiferous tubules of left testes, 35 days following last busulfan injection, while right testis in the group was considered for comparison as controls. Sixty days following transplantation of cell, histomorphometric and histopathologic changes of the experiments were assessed. Results: After AdSCs’ transplantation, normal spermatogenesis appearance was noticed compared to busulfan-induced azoospermia and AdSCs recovered spermatogenesis, and our findings can be added to the literature in treating azoospermic infertilities. Conclusion: The transplanted AdSCs could induce production of germinal cells using testicular seminiferous tubules and were an effective source in treating azoospermia.
Keywords
Adipose Tissue , Stem Cell , Azoospermia , Infertility , Busulfan , Guinea Pig
Journal title
Astroparticle Physics
Serial Year
2018
Record number
2452977
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