Evaluation of Gamma Ray Effects on Total Phenolic Contents, Antioxidants and Antibacterial Activities of Cichorium Intybus L

The efficacy of gamma irradiation is well versed as a method of decontamination for food and herbal resources. In the present study, Cichorium intybus L. roots were irradiated at multiple doses of 1, 2, 4, 6, 8 and 10 kGy by a cobalt-60 irradiator. The irradiated and nonirradiated control samples were evaluated for total phenolic contents, chlorogenic acid assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging properties, ferric reducing power assay and antimicrobial activities. The results revealed that radiation treatment at dose levels of 1, 2 and 10 kGy showed no effects on chlorogenic acid contents, antibacterial activities and scavenging properties. On the other hand, radiation treatment at dose levels of 4, 6 and 8 kGy significantly increased chlorogenic acid contents, antibacterial activities and scavenging properties. Similarly, no effects were observed on the antibacterial activities at radiation dose levels of 1, 2 and 10 kGy, while samples irradiated at 4, 6 and 8 kGy showed significant rise in the antibacterial activities against E.coli. Further, significant, rise in the phenolic contents was recorded at 4, 6 and 8 kGy radiation doses. On the contrary, phenolic contents showed decrease at dose level of 10 kGy. The present study concluded that gamma ray has strong potential to stimulate antioxidant as well as antibacterial activities of Cichorium intybus L.