Diagnosis toxogenic Saxitoxin Cyanobacteria by molecular method in Amir Kalayeh Lagoon–Iran

One of the important cyanobacteria toxins is Saxitoxin poison. It affects nervous and respiratory system. In the event of blooming, some of them produce toxins that threaten the health of water. This poison is one of the most neurotoxins, which feeds on the transmission of nerve signals and the best-known paralytic shellfish toxin (PST). The purpose of this study is quick identification of toxigenic cyanobacteria that produce saxitoxin in the International Amir Kelayeh lagoon using PCR Technique. Twenty water samples of different transect were collected fromAmir Kalayeh lagoon. The DNA was extracted by the modified DNG method kit. Polymerase chain reactions (PCR) test was optimized by the DNA toxogen standard strains Anabaena circinalis (AWQC131C) and then were evaluated for the specificity and sensitivity. Amplicon was cloned in PTZ57R plasmid for sequencing and provide positive control by the T/A cloning method. The PCR test was optimized and 602bp were produced by the standard strains. The Amplicon was cloned and the gene sequence was determined. In specificity survey, no production was produced with TOX cyanobacteria DNA. The sensitivity of the diagnostic method was able to detect 10 copies. The cyanobacteria which produce poison, was potentially observed in 6 out of 20 samples collected from different stations.