New Molecular Aspects of Cardiac Arrest; Promoting Cardiopulmonary Resuscitation Approaches

Introduction: Cardiopulmonary resuscitation (CPR) is a method to improve survival of patients with cardiac arrest. This study aimed to identify the key genes affected five minutes after cardiac arrest, hoping to elevate the efficacy of CPR.Methods: In this bioinformatics study differentially expressed genes of six pigs were downloaded from GEO and screened. The significant and characterized genes were analyzed via calculating fold change and proteinprotein interaction (PPI) networks. The crucial nodes were determined based on centrality parameters and their related biological processes were investigated via ClueGO.Results: 17 significant upregulated (LogFC ≥ 2) and 22 downregulated (LogFC lt; 0.5) genes were detected. Transthyretin (TTR logFC = 4.59) and Gonadotropinreleasing hormone receptor (GNRHR logFC = 3.84) had higher logFC among upregulated and downregulated genes, respectively. The critical genes including four upregulated and five downregulated genes were detected from network analysis. GNRHR and Prolactin precursor (PRL) were among the most important down res 5 minutes after cardiac arrest and Beta2 adrenergic receptor and Cadherin1 were among the most important up regulated gens. Conclusion: The introduced potential biomarkers could reveal a new molecular aspect for CPR performance and pituitary gland protection was highlighted in this respect.