Title of article
Influence of Additives on Fabrication and Release from Protein Loaded Microparticles
Author/Authors
Bhatt, Yagnesh A.P.M.C. College of Pharmaceutical Education and Research, Himmatnagar - Affiliated to Hemchandracharya North Gujarat University, Gujarat, India , Shah, Dushyant A.P.M.C. College of Pharmaceutical Education and Research, Himmatnagar - Affiliated to Hemchandracharya North Gujarat University, Gujarat, India
Pages
9
From page
171
To page
179
Abstract
The purpose of this study was to investigate the effect of additives, poly(ethylene
glycol) (PEG) 1450, poloxamer 407, polyvinyl alcohol (PVA) and sodium chloride
in order to improve physico-chemical characteristics, encapsulation efficiency and
in vitro release of bovine serum albumin, form poly(D,L-lactic-co-glycolic acid)
(PLGA) microparticles prepared by the w/o/w solvent evaporation method. The
addition of PEG 1450 and 0.05 M NaCl changed the surface characteristics of
microparticles and also affected the encapsulation efficiency and burst release of
protein. The effect of surfactants: polyvinyl alcohol and poloxamer 407 used in the
outer water phase was investigated. The surfactant/PLGA mass ratio played an
important role in the preparation procedure of the particles. This ratio was found
to be approximately 0.5 for PVA and 5 for poloxamer 407 in order to achieve
microparticles with narrow size distribution (<70 μm) and good encapsulation
efficiency (>70%).
Keywords
Bovine serum albumin , Encapsulation efficiency , Initial release , Modified Lowry Protein Assays , Poly (lactide-co-glycolide)
Journal title
Astroparticle Physics
Serial Year
2012
Record number
2481780
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