Title of article
Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran
Author/Authors
Davoodabadi ، Abolfazl Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences , Abbaszadeh ، Maryam Department of Molecular Biology - Pasteur Institute of Iran , Oloomi ، Mana Department of Molecular Biology - Pasteur Institute of Iran , Bouzari ، Saeid Department of Molecular Biology - Pasteur Institute of Iran
From page
1
To page
0
Abstract
Background: Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea. Objectives: This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children with diarrhea between 2007 - 2008 in Tehran, Iran. Materials and Methods: EAEC strains were examined for virulence plasmid genes (aap, aggR, and aatA), biofilm formation, and drug resistance. In addition, pulsed-field gel electrophoresis (PFGE) profiles of these strains were determined. Results: Significant percentage of local EAEC strains carried the virulence plasmid genes and formed biofilms. In addition, these strains showed high resistance to ampicillin (100%), tetracycline (65.7%), streptomycin (58.7%), chloramphenicol (52.6%), and trimethoprim/sulfamethoxazole (51.7%) and had different PFGE patterns. Conclusions: These results indicated that EAEC strains isolated from Iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries.
Keywords
Virulence Factors , Drug Resistance , Biofilms , Electrophoresis Gel Pulsed , Field , Escherichia coli
Journal title
Jundishapur Journal of Microbiology (JJM)
Journal title
Jundishapur Journal of Microbiology (JJM)
Record number
2510596
Link To Document