Separation and determination of lead in human urine and water samples based on thiol functionalized mesoporous silica nanoparticles packed on cartridges by micro column fast micro solidphase extraction

An efficient method based on thiol functionalized mesoporous silica nanoparticles (HS-MSNPs) was used for extraction of lead ions (PbII) from urine and water samples by packed column micro solid phase extraction (PC-MSPE). By procedure, 15 mg of HS-MSNPs packed in syringe cartridges (SC, 5mL) with cellulose membrane and pH adjusting at 5.5-6.5. Then, the lead of urine and water sample was efficiently extracted on HS-MSNPs after pushing the plunger of a syringe. Finally, the Pb (II) was back-extracted with inorganic acid solution and the remained solution determined by electrothermal atomic absorption spectrometry (ET-AAS). By optimization conditions, the enrichment factor, LOD, linear range and RSD% was obtained 24.8, 0.04 μg L^-1, 0.12-5.5 μg L^-1 and less than 5%, respectively for 5 mL of urine samples. The validation was confirmed by spiking of real samples and using certified reference material (CRM, NIST) in water and urine sample.