Isolation and identification of antibacterial compound from common name Holothuria leucospilota in the Persian Gulf

Echinodermata are one of the most important branches of invertebrate in marine ecosystems, which today are also known as a rich source of natural products with biological properties. Most of these properties belong to compounds with a terpenoids structure. According to the biological properties of sea cucumbers in the Persian Gulf, this scientific study investigates the antibacterial properties of lanosterol compounds extracted from Holothuria leucospilota in Hengam Island in the Persian Gulf. The sea cucumber powder was extracted using acetone, then the extract purified by silica gel column chromatography with n-hexane and ethyl acetate combination to isolate the terpenoid compounds. Isolated compound were run through TLC and sprayed with vanillin-sulphuric acid reagent for detection of terpenoid compounds and profile of isolated compounds obtained by gas chromatography (GC). The lanosterol teriterpenoids compound in column fraction was identified by GC. Antimicrobial properties were evaluated by dilution test on bacterial strain Escherichia coli, Pseudomonas aeruginosa, Klebsiella nemonya, Proteus vulgaris, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and Nocardia brazilinus. This compound show antibacterial activities against gram-positive bacteria (B. subtilis, B. cereus and S. aureus). Antibacterial results of lanosterol authenticated that could be an efficacious compound in antibiotics production