Title of article
Overexpression of molecular chaperons GRP78 and GRP94 in CD44hi/CD24lo breast cancer stem cells
Author/Authors
Nami Babak Department of Medical Genetics and The Women and Children’s Health Research Institute (WCHRI) - University of Alberta - Edmonton - Alberta, Canada , Ghasemi-Dizgah Armin Department of Microbiology Iran University of Medical Sciences - Tehran, Iran , Vaseghi Akbar Young Researchers and Elite Club - Ardabil Branch - Islamic Azad University - Ardabil, Iran
Pages
6
From page
105
To page
110
Abstract
Breast cancer stem cell with
CD44hi/CD24lo phonotype is described
having stem cell properties and represented
as the main driving factor in breast cancer
initiation, growth, metastasis and low
response to anti-cancer agents. Glucoseregulated
proteins (GRPs) are heat shock
protein family chaperons that are charged
with regulation of protein machinery
and modulation of endoplasmic reticulum homeostasis whose important roles in stem cell
development and invasion of various cancers have been demonstrated. Here, we investigated
the expression levels of GRP78 and GRP94 in CD44hi/CD24lo phenotype breast cancer stem cells
(BCSCs).
Methods: MCF7, T-47D and MDA-MB-231 breast cancer cell lines were used. CD44hi/CD24lo
phenotype cell population were analyzed and sorted by fluorescence-activated cell sorting
(FACS). Transcriptional and translational expression of GRP78 and GRP94 were investigated by
western blotting and quantitative real time PCR.
Results: Results showed different proportion of CD44hi/CD24lo phenotype cell population in
their original bulk cells. The ranking of the cell lines in terms of CD44hi/CD24lo phenotype cell
population was as MCF7
Keywords
Breast cancer , Cancer stem cell , GRP78 , GRP94 , Overexpression
Journal title
Bioimpacts
Serial Year
2016
Record number
2515820
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