Title of article
Probable Role of Bilophila wadsworthia in Appendicle Infection
Author/Authors
Tabatabaei, Mohammad Assoociated Professor Microbiologist - Faculty of Veterinary Medicine - Shiraz University , Dehvari, Aslam M.Sc. student in bacteriology - Faculty of Veterinary Medicine - Shiraz University , Geramizadeh, Bita hiraz Transplant Research Center - Shiraz University of Medical SciencesShiraz , Niakan, Mohammad Hadi Trauma Research Center - Shiraz University of Medical Sciences
Pages
7
From page
29
To page
35
Abstract
Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens.
Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers.
Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.
Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues.
Keywords
Bilophila spp. , Appendectomy , Appendicle specimens , PCR , Nucleotide sequencing
Journal title
Medical Laboratory Journal
Serial Year
2020
Record number
2525800
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