Maternal donors of polyploids in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from chloroplast trnL-F sequences

To investigate the maternal donors and phylogenetic relationships of polyploids in Pseudoroegneria and related genera, the chloroplast trnL-F sequences of 31 Triticeae accessions were analyzed. The substitution saturation analysis of trnL-F sequences in this study suggested that they are suitable for phylogenetic analysis. In the trnL-F sequences tree, 4 major clades were formed: (a) the St/E clade comprised all of the Pseudoroegneria species together with species in Roegneria, Elytrigia, Douglasdeweya and Lophopyrum; (b) A. cristatum, A. mongolicum, and A. pectinatum subsp. retrofractum formed the P/W clade; (c) the Ns clade included species in Psathyrostachys; and (d) the H clade consisted of Hordeum species. The results suggested that: (a) diploid Pseudoroegneria species were the maternal donors of Stcontaining polyploid species in Pseudoroegneria, Roegneria, Douglasdeweya, and Elytrigia, and the trnL-F sequences were highly similar among them; (b) the trnL-F sequences of Agropyron species and Australopyrum species were similar, and the P genome was closely related to the W genome; and (c) the trnL-F sequences of species with the H or Ns genomes diverged greatly from that of species with the St, E, P, or W genomes.