Title of article
Comparative analysis of DNA extracted from fish species preserved in formalin in two diff erent periods
Author/Authors
RAJA, Manickam Manonmaniam Sundaranar University - Sri Paramakalyani Centre for Environmental Sciences, INDIA , MURALIDHARAN, Murugan Manonmaniam Sundaranar University - Sri Paramakalyani Centre for Environmental Sciences, INDIA , ARUNACHALAM, Muthukumarasamy Manonmaniam Sundaranar University - Sri Paramakalyani Centre for Environmental Sciences, INDIA
From page
331
To page
336
Abstract
DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the viability of extracting genomic DNA from formalin preserved fish specimens and to estimate the quality and quantity.
Keywords
Cyprinid fishes , formalin preserved tissue , DNA extraction , quality analysis
Journal title
Turkish Journal of Biology
Journal title
Turkish Journal of Biology
Record number
2533981
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