Title of article
Frequency of bovine lymphocyte antigen DRB3.2 alleles in Sarabi cows
Author/Authors
Montazer Torbati, Fatemeh ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Eftekhari Shahroudi, Fereidoun ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Nassiry, Mohammad Reza ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Safarnezhad, Abbas Jahad-Keshavarzi Research Center, ايران , Montazer Torbati, Mohadmmad Bagher university of tehran - Agriculture Faculty - Department of Animal Science, تهران, ايران
From page
101
To page
105
Abstract
The second exon of the bovine Major Histocompatibility Complex (MHC) class II DRB3 gene was amplified by polymerase chain reaction (PCR) from 50 DNA samples of Iranian Sarabi cattle. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, BstyI, and HaeIII was conducted on DNA from samples. Fifteen Bovine Lymphocyte Antigen (BoLA)-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.23. The most frequent alleles were *52 (frequency = 0.23), *11 (0.18) and *23 (0.15). Results of this study demonstrate that the BoLA-DRB3.2 locus is highly polymorphic in Sarabi cattles.
Keywords
BoLA , DRB3.2 , polymerase chain reaction (PCR) , restriction fragment length polymorphism (RFLP) , Iranian Sarabi cows.
Journal title
Iranian Journal of Biotechnology (IJB)
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
2549030
Link To Document