Antibacterial, antifungal and cytotoxic activities of 3,5- diacetyltambulin isolated from Amorphophallus campanulatus Blume ex. Decne

Background and purpose of the study: Amorphophallus campanulatus is widely distributedin Bangladesh, India, and Africa and the tuberous roots of the plant has many traditionaluses and is an important source of biologically active compounds. In the present study invitro antibacterial, antifungal and cytotoxic activities of 3,5-diacetyltambulin which is aflavonoid isolated from Amorphophallus campanulatus was studied.Materials and Methods: In vitro antibacterial and antifungal activities was evaluated by discdiffusion and MICs technique was determined by serial dilution technique. Cytotoxicitywas determined against brine shrimp nauplii.Results and Major conclusion: The compound showed significant antibacterial activitiesagainst four Gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcusaureus, Streptococcus β-haemolyticus) and six Gram-negative bacteria (Escheichia coli,Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonus aeruginosa,Salmonella typhi). The MIC values against these bacteria ranged from 8 to 64 μg/ml buthad weak antifungal activity against a number of fungi. In cytotoxicity determination, LC50of the compound against brine shrimp nauplii was 10.02 μg/ml.