Validation and Uncertainty Estimation of Analytical Method for Determination of Benzene in Beverages

Analytical method for the determination of benzene in beverages was developed using HS/GC-FID technique. Validation of the method was done for knowing the performance of the analytical method used. Linearity obtained r^2= 0.9971 was observed in the range from 5 to 100 ng mL^-1 of benzene in sample. The method has good recoveries (average 101 %) and average of relative standar deviation for repeatability and intermediate precision at 3 level concentrations are less than 10 %. The limit of detection and limit of quantitation obtained from calculation are 0.90 and 2.86 ng mL^-1 respectively. The expanded uncertainty of the method, U, is about 30 %.