Title of article
Molecular Phylogeny of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) as Inferred from Cytochrome C Oxidase I Mitochondrial Dna Gene Sequences
Author/Authors
KAMARUDIN, KAMARUL RAHIM The University of Auckland - School of Biological Sciences (SBS), New Zealand , Rehan, Aisyah Mohamed International Islamic University Malaysia (IIUM) - Kulliyyah of Science - Institute of Oceanography Maritime Studies (INOCEM), Malaysia , Ridzwan, Hashim International Islamic University Malaysia (IIUM) - Kulliyyah of Science - Institute of Oceanography Maritime Studies (INOCEM), Malaysia , Gires, Usup Universiti Kebangsaan Malaysia - School of Environmental Natural Resource Sciences,Faculty of Science Technology, Malaysia , Fauzan Ahmad, Hajar International Islamic University Malaysia (IIUM) - Kulliyyah of Science - Institute of Oceanography Maritime Studies (INOCEM), Malaysia , Hanafi Anua, Mohd International Islamic University Malaysia (IIUM) - Kulliyyah of Science - Institute of Oceanography Maritime Studies (INOCEM), Malaysia , Yaman Idris, Mohd International Islamic University Malaysia (IIUM) - Kulliyyah of Science - Institute of Oceanography Maritime Studies (INOCEM), Malaysia
From page
125
To page
133
Abstract
Holothuria (Mertensiothuria) leucospilota (Brandt 1835), white threads fish or locally known as bat puntil is currently considered as the most abundant sea cucumber species in Malaysia. This study aimed to generate the genetic profile of H. leucospilota from Malaysia and then to determine the phylogenetic relationship between H. leucospilota and other members of genus Holothuria using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Kedah Darul Aman in the west coast of Peninsular Malaysia. Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for the phylogenetic tree reconstruction. Tree topologies showed that H. leucospilota has its own monophyletic clade clearly distinct from the other species. The pairwise genetic distance calculated further supported these findings. In addition, the results also should that the COI mtDNA gene is capable to unravel the phylogenetic relationship of H. leucospilota.
Keywords
Holothuria leucospilota , molecular systematics , partial sequences of cytochrome oxidase I gene , phylogeny tree
Record number
2555047
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