COMBINED EFFECTS OF 50 HZ ELECTROMAGNETIC FIELD AND SIO2 NANOPARTICLES ON OXIDATIVE STRESS IN PLANT’S GAMETIC CELLS

The paper presents the results of combined effects of an extremely low frequency electromagnetic field (50 Hz) (ELF EMF) and SiO2 nanoparticles on fluorescence of plant gametic cells (immature microspores). The data were recorded by a BD FACSJazz® cell sorter after cell irradiation by blue laser (488 nm). A significant difference of fluorescence was observed between gametic cells after 1 hour incubation in suspension of SiO2 nanoparticles and the control gametic cells. It was observed that fluorescence intensity of gametic cell was higher with ELF EMF treat- ment in comparison to control cells, but it was statistically significant only for cells treated with electromagnetic radiation field with density 100 µT (p 0.01) and 400 µT (p 0.01). A different effect of ELF EMF were observed in cells incubated in SiO2 nanoparticle suspension; interaction of the factors resulted in lower cell fluorescence in comparison to control cells. The present study showed that SiO2 nanoparticles may be a source of oxidative stress, but jointly with 50 Hz elec tromagnetic field they can serve as an efficient antioxidant.