Sensitive LC method for the simultaneous determination of diacerein and aceclofenac in tablet dosage form

Background: Diacerein and aceclofenac drug product combination is used for the pain management in osteoarthritis. The main aspects of the present study is to develop stability indicating, sensitive reverse phase high-performance liquid chromatographic method for the simultaneous determination of diacerein and ceclofenac in tablet dosage form. Were, the chromatographic separation was achieved on Kromasil C-18, 150 × 4.6 mm, 3.5-μm analytical column using mobile phase double distilled water (pH 2.7 with glacial acetic acid)-acetonitrile (45:55 v/v). Detector was set at 256 nm. Results: The described method shows excellent linearity over a range of 75–2.5 μg/mL for diacerein and 150–5 μg/mL for aceclofenac. The correlation coefficient for diacerein is 0.9999 and for aceclofenac is 0.9997. Conclusions: The proposed method was found to be suitable for simultaneous determination and stability study of diacerein and aceclofenac in tablet dosage form.