Statistical optimisation of radial growth rates of Ganoderma neo-japonicum (KLUM61076) in low cost solid agar plate’s medium using full factorial design and central composite design

The combination of low cost medium sources (brown sugar, spent yeast, molasses and corn steep liquor) for growth of Ganoderma neo-japonicum (KLUM61076) in solid agar plates was studied. The mathematical model of Full Factorial Design and Response Surface Methodology were used to select the optimum combination of carbon/nitrogen sources for the cultivation of G. neo-japonicum. The combination medium of brown sugar and spent yeast was the best for growth of G. neo-japonicum at 5.74% (w/v) carbon content in brown sugar and 0.06% carbon content (w/v) in spent yeast. The combination of two constituents contributed to a low requirement of C/N ratio 1.74 and gave the fastest mycelial growth rates (20.74 mm/day). This combinations of spent yeast/brown sugars showed an increment in mycelia yield, which is up to two-fold as compared with the standard commercial medium (yeast extract-glucose). The low cost medium has the potential to contribute to cost-effective cultivation for G. neo-japonicum large-scale production.