Title of article
In-vitro propagation of Pluchea lanceolata (DC) C.B.Clarke a potent antiarthritic medicinal herb through axillary bud
Author/Authors
Singh ، Satendra Division of Genetics and Plant Breeding - CSIR-Central Institute of Medicinal and Aromatic Plants , Mishra ، Ritu Division of Genetics and Plant Breeding - CSIR-Central Institute of Medicinal and Aromatic Plants , Agnihotri ، RAJNEESH K. Department of Botany - School of Life Science - Dr. B. R. Ambedkar University, Khandari Campus
From page
24
To page
30
Abstract
Pluchea lanceolata, commonly known as Rasana, is highly valued for its antiinflammatory, antiarthritic, and analgesic properties and is extensively employed in treating rheumatoid arthritis, cough, neurological diseases, edema, sciatica, psoriasis, bronchitis, dyspepsia, and piles. Shrinking populations and insufficient variation due to rootstock propagation methods warrant efficient mass multiplication and conservation protocols. In the present study, explant (axillaries bud was cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxin (PGRs) individually, as well as in combination. The highest mean shoot number (2.06 ± 1.43) and second highest mean shoot length (2.62 ± 1.49 cm) were obtained in MS media enriched with 4 mg/L Benzyl amino purine (BAP) + 1mg/L Naphthalene acetic acid (NAA). Whereas, the best shoots multiplication (2.62 ± 1.41) and shoots elongation (5.88 ± 2.44) responses were displayed when MS medium supplemented with 0.25 mg/L (BAP) +0.50 mg/L Kinetin (KIN) were used.
Keywords
Axillaries bud explant , In , vitro propagation , MS medium , PGRs , Pluchea lanceolata
Journal title
Trends In Phytochemical Research
Journal title
Trends In Phytochemical Research
Record number
2581073
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