In Vitro Effect of Probiotic Mix and Fibrolytic Enzyme Mixture on Digestibility of Paddy Straw

The study was undertaken to evaluate the effects of probiotic mix and fibrolytic enzyme mixture on in-vitro fermentation and digestibility using whole rumen flora from sheep. The feed additives viz. probiotic mix and fibrolytic enzyme mixture were incorporated @ 3g, 6g, 9g, 12g, 15g/kg dry matter of complete feedfor adult sheep. The rations were subjected to in- vitro analysis to evaluateDM, OM and NDF digestibilityfor determination ofoptimum level of incorporation of feed additives in the complete ration.Significant improvement in nutrient degradability of DM, OM and NDF was observed due to probiotics mix and fibrolytic enzyme mixture supplementation with maximum values at 3g and 9g kg-1DM, respectively. Another in vitro study was carried to evaluate the effect of 3g and 9g kg-1 DM of probiotics mixand fibrolytic enzyme mixture alone and in combination on rumen fermentationparametersin complete feed having paddy straw and concentrate mixture in 50:50 ratio. The probiotics mixand fibrolytic enzyme mixture alone and in combination significantly improved nutrient degradability (DM, OM and NDF), total rumen nitrogen and TVFA in comparison to control. Additions of probiotic mix in complete feed decreased rumen pH alone and in combination with enzyme mix with a significant decrease in rumen ammonia nitrogen.