PCR Based Detection of Nosocomial Infection Causing MRSA (Methicillin Resistant Staphylococcus Aureus)

Nosocomial infections are infections that develop as a result of a stay in hospital or are produced by microorganisms and viruses acquired during hospitalization. Nasal carriage of S. aureus is very common and may be due to hand to nose transmission. The objective of the present study was to screen individuals from various sectors as carriers of MRSA. The study group included hospital staff such as nurses, doctors and visitors. Nasal swabs were taken from the volunteers and cultured on media selective for Staphylococcus aureus. Pathogenecity was confirmed using PCR for the coagulase gene. Coagulase positive strains were subjected to PCR for the mec gene. A comparison was done with a student population to serve as a group non exposed to MRSA.