Effect of Different Doses from ZnONPS on the Pituitary-Testes Axis Function in Adult Male Rats

In our study, ZnONPs was prepared and characterized for investigation of pituitary-testes axis function in adult male rats. Fifty healthy adult male rats selected, weighted 300 ± 50 gm, randomly divided to five group each group have ten animals. One of them group consider as control group and other four group designed as (G1, G2, G3 and G4) group which receive oral dose of ZnONPs in different concentration (5, 10, 20 and 40 mg/kg- B.W) respectively for 56 day. Blood samples were collected via cardiac puncture for serum collection in (zero time, 28 and 56 day of experiment). The results showed significant (P<0.05) elevation of testosterone and FSH hormones concentration in G3 and G4 after 56 day of experimental period as a compared with zero time and 28 day of experiment, whereas LH and inhibin B hormones concentration showed a significant (P<0.05) decrement in all group at 56 day as a compared with control group and other period time. Histological study of testes and pituitary gland showed different pathological changes on these two gland’s tissue and cells as mild to severe effects especially at dose (20 and 40 mg/kg B.W). Histometric study of seminiferous tubules illustrated a significant (P<0.05) elevation of seminiferous tubules diameters in G4 group, whereas showed non-significant (P˃0.05) differences in number of interstitial cells in treated groups.