Title of article
Diagnosis of Canine Leishmaniasis using Ear Blood PCR in Region of Brazil
Author/Authors
Neitzke-Abreu, Herintha Programa de Pós-Graduação em Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , Duarte Vieira, Victor Bruno Programa de Pós-Graduação em Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , Stefanello da Silveira, Ana Paula Programa de Pós-Graduação em Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , Surian Batalini, Leticia Faculdade de Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , Gnutzmann, Laísa Vieira Programa de Pós-Graduação em Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , Oliveira Castro, Silvana de Faculdade de Ciências da Saúde - Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil , do Carmo, Silvia Barbosa Control Center of Zoonosis (CCZ), Campo Grande, MS, Brazil , Friozi, Elisabete Control Center of Zoonosis (CCZ), Campo Grande, MS, Brazil , Costa Lima-Junior, Manoel Sebastião da Fundação Oswaldo Cruz (FIOCRUZ) - Instituto Aggeu Magalhães, Recife, PE, Brazil
Pages
4
From page
157
To page
160
Abstract
The objective of this study was to evaluate a polymerase chain reaction (PCR) for the diagnosis of Canine
leishmaniasis (CanL) using less invasive sample collection. PCR-based detection of Leishmania infantum using ear
peripheral blood collected from dogs with symptomatic CanL and coinfection with Ehrlichia sp. in the city of Campo
Grande (Brazil) was investigated. A total of 268 CanL seropositive dogs were sampled. Of the 268 dogs tested, 70.90%
were PCR positive for CanL, 72.43% of the dogs with ear lesion/s (185) were PCR positive, whereas only 67.47%
of dogs without ear lesion/s (83) were PCR positive. Additionally, 35.82% of dogs tested were positive based on
parasitological diagnosis for Ehrlichia sp. It was concluded that ear blood PCR may be an alternative for the diagnosis
of CanL. Because it is more rapid collect and less invasive sample, this makes ear-blood PCR a potentially useful
supplement to traditional diagnostic methods.
Keywords
Dog , Ehrlichia , Peripheral blood , Leishmania infantum , Co-infection
Journal title
Advances in Animal and Veterinary Sciences
Serial Year
2020
Record number
2591023
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