Stocking Density Associated with Parasitic Infection by Gastrointestinal Examination in Laboratory Mice

Parasitic infection in the laboratory mice (Mus musculus) may affect certain research outcomes especially during high worm burden. This study allows the assessment of parasite infection of mice based on stocking density that potentially serve as a guideline for proper management to be implemented. Fifty-four (54) male BALB/c mice were randomly chosen from an animal facility located in Klang Valley, Malaysia and placed for three replicates in groups of 3, 6 and 9 mice per cage to reflect different stocking densities. Endoparasites were examined by direct faecal smear, perianal tape test, faecal floatation and gastrointestinal examination techniques. Ectoparasites were identified under fur pluck method, tape impression test and carcass immersion while blood smear techniques were performed for blood parasites detection. Samples were taken weekly for a total of 5 weeks. Results revealed infection with pinworms; Syphacia obvelata and Aspiculuris tetraptera. Statistical analysis revealed no association between parasites and various stocking density using repeated-measures ANOVA. However, gastrointestinal examination mentioned as the ‘gold standard’ revealed an association using one-way ANOVA when P < 0.05. Overall, the results varied according to the parasitological methods used and stocking densities do not play a role in the parasitic levels of laboratory mice