Pathogenic activity of Fusarium equiseti from plantation of Citrus Plants (Citrus nobilis) in the village Tegal Wangi, Jember Umbulsari, East Java, Indonesia

Some fungi associate with fruit and dead or dying plant tissues as pathogen on a wide range of agricultural plants. This work comprised the isolation, identification and pathogenic assay from citrus fruit plantations (Citrus nobilis), Tegal Wangi, Jember, Jawa Timur, Indonesia with 34 mold isolates obtained. Color of 7-day-old colonies cultures on PDA was dominated by white while the reverse was whitish to pale yellow. Based on the pathogenicity test, four representative mold isolates were identified as pathogenic fungi using the sequence of internal transcribed regions Spacer (ITS) in the region of ribosomal DNA selected. Molds were identified as UNJCC (D5) D5K3A (Fusarium equiseti with 98% homology bootstrap value 100%), UNJCC (D6) D6. K3.B (F. equisetiwith 99% homology bootstrap value of 100%), and UNJCC (D7) D7.K2.B (F. equisetiwith 99% homology bootstrap value 66%) and UNJCC (D8) D3.K2.B (F. equisetiwith 99% homology bootstrap value of 55%). F. equisetiis a main source of trichothecenes, zearalenone and other mycotoxins which can cause serious disease in humans and animals. Present information regarding the Fusariumequiseti damage to citrus leaves can be used help identify the occurrence of pathogenic fungi in citrus fruit plantations.