Title of article
Thermodynamic Analysis of Myelin Basic Protein Adsorbed onLiquid Crystalline Dioleoylphosphatidylcholine Monolayer
Author/Authors
Chengxi, Hu Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Lei, Zhang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Huihui, Yang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Runguang, Sun Schools of Physics and Information Technology - Shaanxi Normal University - Xi’an - Shaanxi, China , Changchun, Hao Schools of Physics and Information Technology - Shaanxi Normal University - Xi’an - Shaanxi, China , Huihui, Yang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China
Pages
10
From page
1
To page
10
Abstract
Toinvestigatethestabilityanddynamiccharacteristicsofmonolayeradsorbedonunsaturatedlipiddioleoylphosphatidylcholine (DOPC) withvaryingconcentrationsofmyelinbasicprotein(MBP), thesystemisstudiedbyapplyingLangmuirtechniqueandmakingatomicforcemicroscope(AFM)observation,whichisbasedonthemassconservationequationanalysismethodreferredtointhethermodynamicstheory.Asindicatedbysurfacepressure-meanmoleculararea (π−A) and surfacepressure-adsorptiontime(π−T) isotherms,thephysicalpropertiesofmonolayerderivedfromtheinteractionofvaryingconcentrationsofMBPwithliquidcrystallineunsaturatedlipid DOPCmoleculeswerequalitativelystudied.AsrevealedbysurfacemorphologyanalysiswithAFM,themicroregionwasexpandedastheconcentration of MBPinthesubphasewasontheincrease,suggestingthathydrophobicinteractionsledtotheMBPinsertion,thuscausingaccumulationoftheMBPonthesurfaceofthemonolayer. Experimentalresultshavedemonstratedthatthepartitioncoefficientoftheinteractionbetween MBP andunsaturatedphospholipid DOP CandthemolecularareaofMBPadsorbedonthemonolayerfilmwascalculatedusingthemassconservationequation.Inaddition,notonlydoesthevaryingconcentrationofMBPinthesubphaseexertssignificanteffectsonthearrangementandconformationof DOPCmonolayer,italsohascertainguidingsignificancetoexploringthestructuralchangestobiofilmsupramolecularaggregatesaswellasthepathogenesisandtreatmentofrelateddiseases.
Keywords
Thermodynamic Analysis , Myelin Basic Protein Adsorbed , Liquid Crystalline Dioleoylphosphatidylcholine Monolayer , AFM , DOPC
Journal title
Scanning
Serial Year
2019
Record number
2613392
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