Title of article
Derivatization of Aminoglycoside Antibiotics with Tris(2,6-dimethoxyphenyl)carbenium Ion
Author/Authors
Topolyan, A.P. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Belyaeva, M.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Bykov, E.E. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Coodan, P.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Rogozhin, E.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Strizhevskaya, D.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Ivanova, O.M. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Ustinov, A.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Mikhura, I.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Prokhorenko, I.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Korshun, V.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Formanovsky, A.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia
Pages
8
From page
128
To page
135
Abstract
Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6-dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures.
Farsi abstract
فاقد چكيده فارسي
Keywords
aminoglycoside antibiotics , mass-spectrometry , trityl cation , HPLC
Journal title
Acta Naturae
Serial Year
2016
Full Text URL
Record number
2616106
Link To Document