A new concept for production and scaling up of bioactive compounds from Egyptian date palm (Zaghlool) cultivar using bioreactor

A promising and successful protocol for enhancement and production of total phenolic and peroxides compounds from Egyptian date palm cultivar Zaghlool cells in stirred tank reactor was established. The influence of cell culture cultivation in combination with Aspergillus niger extract, and methyl-jasmonate elicitors incorporation in the feeding medium on cell growth patterns and production of active compounds was investigated. The maximum value of cell growth parameters and the highest content of bioactive compounds were obtained from elicitation of modified MS-medium with Aspergillus niger extract at 0.1% in combination with methyl-jasmonate (100 μM), as compared with other concentrations after 10 days of cultivation. The chemical analyses of phenolic and peroxidase compounds were spectrophotometrically performed.