• Title of article

    Long non-coding RNA expression profiling in the lesional tissue and derived fibroblasts of keloid

  • Author/Authors

    Yuan,Chunyu Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Bu, Wenbo Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Li, Li Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Zhang, Mengli Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Chen, Kun Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Fang, Fang Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Li, Min Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Chen, Xu Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China , Gu, Heng Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking unio‎n Medical College, Nanjing, China

  • Pages
    14
  • From page
    587
  • To page
    600
  • Abstract
    Introduction Long non-coding RNA (lncRNA) plays a key role in various disorders. However, its role in keloid is still unclear. Aim We explored differentially expressed (DE) lncRNAs and mRNAs between keloid tissue (KT)s and normal tissue (NT)s, as well as keloid fibroblast (KFB)s and normal fibroblast (NFB)s, respectively. Material and methods We use KTs and NTs from the chest of 5 patients, and 3 pairs of KFBs and NFBs, to perform microarray respectively. Gene ontology and pathway analyses were conducted by online software DAVID (Database for Annotation, Visualization and Integrated Discovery). The validation of targeted lncRNAs were conducted by qRT-PCR in enlarged samples (79 KTs and 21 NTs). Results We identified 3680 DE-lncRNAs in tissue essay, and 1231 DE-lncRNAs in cell essay. Furthermore, we found that many lncRNAs and their relative mRNAs were regulated simultaneously in keloid. We identified that ENST00000439703 and uc003jox.1 were up-regulated in both of the above essays through comparing the results of lncRNA screening between tissue essay and cell essay; the results were confirmed through qRT-PCR in enlarged samples. Conclusions Our study demonstrates that numerous lncRNAs are involved in the pathogenesis and development of the keloid.
  • Keywords
    long non-coding RNA , keloid , fibroblast , mRNA , pathway
  • Journal title
    Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii
  • Serial Year
    2017
  • Full Text URL
    www.ncbi.nlm.nih.gov/pmc/articles/PMC5799763/pdf/PDIA-34-31388.pdf
  • Record number

    2623038

    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=10&DC=2623038