Title of article
PURIFICATION OF KLEBOCIN EXTRACTED FROM LOCAL ISOLATE Klebsiella pneumonia (K64)
Author/Authors
Musleh, Rashid M. University of Baghdad - College of sciences - Department of biology, Iraq , Zahid, Lina A. Omar University of Baghdad - College of sciences - Department of biology, Iraq , AL-Jumaily, Essam F. Baghdad University - Institute of Genetic Engineering and Biotechnology for postgraduate studies, Iraq
From page
239
To page
247
Abstract
Klebsiella pneumonia (K64) isolated from urine was induced by mutagenic agent (Mitomycim C) to produce Klebocin. The Klebocin was extracted and ultra filtrated then purified by Ion-exchange chromatography. Klebocin was obtained by Gel-filtration chromatography; the purified protein was applied to SDS-PAGE gel electrophoresis. The antimicrobial activity was tested against different microorganisms that included: Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Candida albicans and Aspergillus niger. The Klebocin partially purified from its immunity protein by ion exchange chromatography and completely purified by gel filtration chromatography. The purity was confirmed from one band obtained from electrophoresis. Staphylococcus aureus was very sensitive while (K72) K.pneumonia revealed a minimum inhibition zone.
Keywords
Klebocin , Klebcin (K64)
Journal title
iraqi journal of biotechnology
Journal title
iraqi journal of biotechnology
Record number
2636963
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