The Ability of Rapeseed (brassica napus l.) Seeds Oil in Inhibiting Cell Line Growth in Vitro.

The aim of the present study was assess the cytotoxic activity of seed oil on two cell lines (RD and L20B), through different exposure time (24 , 48 and 72 hrs.) and different concentration of seed oil (1.3 , 2.6 , 5.2 , 10.4 , 20.8 , 41.6 , 83.2 and 166.4 μg / ml) in vitro. The results showed that rapeseed ( Brassica napus L.) seeds oil has toxic effect on RD cancer cell line at high concentrations (83.2 and 166.4 μg/ ml) during 48 and 72 hrs. in comparison with the control. On the other side the result showed that there is no significant effect of these seed oil on the L20B transformed cells, except the treatment with higher concentration (166.4μg/ml) after 48 hrs. exposure time. In conclusion, the oil was shows selective (cell line – dependant) inhibitory effect on cancer cell lines and the plant may be a promising anticancer drug. Other biological properties should be appeared through the use of this oil extracted from the seeds of rapeseed.