Rht-B1b and Rht-D1b analysis of Semidwarf wheat cv. Uruq produced by Induced Mutation

there are many genes associated with dwarfing growth in bread wheat. Two genes in particularRht-B1b and Rht-D1b were used for identification of dwarfism in many wheat varieties. In this study PCR technique was used for identification of Rht-B1b and Rht-D1b in parent cv. Inia-66 and mutant genes in semidwarf cv. Uruq. Two sets of primers were used for identification of mutants genes in semidwarf cv. Uruq and its parent Inia-66 genes. The results showed that cv. Uruq contains the dwarf gene Rht-D1 which is absent in the parent cv.Inia-66. RAPD-PCR performed in order to establish a molecular marker for cv. Uruq. A specific band was observed in cv. Uruq but absent in cv. Inia-66. These results proved that Uruq is semidwarf wheat at the molecular level.