Title of article
RT PCR Detection and Propagation of Respiratory Syncytial Virus in Human Lung Carcinoma Cells (A549) cell line
Author/Authors
ali, layla f. university of baghdad - college of science - department of biology, Iraq , al-suhail, raghad g. university of baghdad - college of science - department of biology, Iraq , naser, faisal g. ministry of health - virology department, central public health laboratory, Iraq
From page
69
To page
74
Abstract
In this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients hospitalized with signs and symptoms of an upper respiratory tract infection in Central Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples.
Keywords
RT PCR , RSV , Tissue culture.
Journal title
Iraqi Journal Of Science
Journal title
Iraqi Journal Of Science
Record number
2638803
Link To Document