Evaluating the Genetic Relatedness within Lupinus pilosus L. Species Based on RAPD Analysis

Nine Random Amplified Polymorphic DNA (RAPD) primers showing polymorphic bands were used to determine the genetic relatedness among and within single individual plants of Lupinus pilosus and used for the construction of the dendrogram and the similarity matrix. A total of 1112 bands were obtained, 219 of which were polymorphic. Similarity values among the studied single individuals of Lupinus pilosus ranged from 0.450 to 0.02. The cluster analysis obtained by Unweighted Pair-Group Method with arithmetic averages (UPGMA) grouped the tested individuals into three clusters. A genetic variation was found within Lupinus pilosus based on RAPD markers. The variability emphasis the presence of genetic diversity within Lupinus pilosus species.