IN VITRO AND IN VIVO STUDY: BY USING OF He-Ne LASER AS PROTOSCOLICIDAL AGENT OF ECHINOCOCCUS GRANULOSUS

Hydatid protoscolices that obtained from sheep infected with Echinococcus granulosus, were irradiated in culture medium in vitro with He-Ne Laser (632.8 nm, 0.5 mW) for different periods (0, 5, 15, 30, 60, and 120) min, and energy densities (0, 1.19, 3.58, 7.17, 14.33, and 28.66) J/cm2. The percentage of the protoscolices viability was assessed after the incubation at temperature 20oC and pH 7.4 at the time intervals (1 hour, 1 day, 4 days, 6 days, and 12 days) after each irradiation period. It was seen that over the time intervals up to 4 days, the irradiation periods (60 and 120) min, and the energy densities (14.33 and 28.66) J/cm^2 reduced the viability in irradiated groups as compared with zero time which was considered as control, whilst all the irradiation periods had no reliable effect at the incubation interval 1 hour. In vivo the results also revealed a noted reduction in the cysts numbers and their diameters at different irradiation periods, and no cysts were found in albino mice incubated with culture irradiated for 120 min