Title of article
DETERMINATION OF PENDIMETHALIN HERBICIDE IN WATER AND VEGETABLE SAMPLES BY MICROWAVE-ASSISTED SOLVENT EXTRACTION AND HPLC METHOD
Author/Authors
Shehzad, Farhat-Un-Nisa University of Peshawar, - College of Home Economics, Pakistan , Shah, Jasmin University of Peshawar - Institute of Chemical Sciences, Pakistan , Jan, M. Rasul University of Peshawar - Institute of Chemical Sciences, Pakistan
From page
175
To page
185
Abstract
A method for High Performance Liquid Chromatography (HPLC) for the residue determination of pendimethalin herbicide in water and food samples was developed. The chromatographic analysis was carried out, on a C18 packed capillary column with gradient elution, 20 μl injection volume and ultraviolet detector at 240 nm. Samples were spiked with amount between 5μg g-1 and 15μg g-1 of herbicide and were isolated from samples by applying microwave assisted extraction (MAE) acetone, ethanol and water as extraction solvent. Percent recoveries were improved by optimizing solvent types, solvent volume and time. Calibration curve range determined by HPLC was 0.2-40μgm L-1. The interaction of different variables for maximum % recovery response was found to be in range from 91.22±0.01-99.32±0.01. Application of this procedure to the analysis of herbicide in water and vegetable samples showed the effectiveness of the methodology proposed.
Keywords
MAE , pendimethalin , water , vegetable samples , HPLC
Journal title
Pakistan Journal of Weed Science Research
Journal title
Pakistan Journal of Weed Science Research
Record number
2648471
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